Regulation of Subunit Synthesis of Escherichia coli RNA Polymerase
- 1 September 1973
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 70 (9) , 2651-2655
- https://doi.org/10.1073/pnas.70.9.2651
Abstract
LambdacI857s7drif(d)18 phage is a defective, specialized transducing phage carrying the RNA polymerase mutation rif(d), a dominant rifampicin-resistant allele. This phage DNA directs in vitro synthesis of the beta subunit of RNA polymerase in a DNA-dependent protein-synthesizing system. Prophage induction in cells that are lysogenic for this phage increases the rate of beta-subunit synthesis in vivo by at most 2-fold. No increase in subunit synthesis above the haploid level is observed in cells diploid for the beta gene.Keywords
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