Abstract
Pacific herring, Clupea harengus pallasi, were collected at three locations in Alaska and Japan in winter and spring to determine their degree of freezing resistance. Herring collected from waters whose temperatures ranged between 4.7 and −1.4 °C showed serum freezing points between −1.22 and −1.40°C. All freezing points were below those expected from measurements of serum osmolality, indicating that a noncolligative antifreeze was present that added between 0.28 and 0.61° to the freezing point depression. In addition, osmotically active substances other than sodium chloride contributed to the freezing point depression. in some of the samples.

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