.beta.-Cyclodextrin-bonded silica for direct injection analysis of drug enantiomers in serum by liquid chromatography
- 1 May 1990
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 62 (10) , 997-1000
- https://doi.org/10.1021/ac00209a006
Abstract
A new .beta.-cyclodextrin (CD) bonded silica has been designed for direct injection analysis of drug enantiomers in serum for liquid chromatography. The new .beta.-CD bonded silica is synthesized by three steps: introduction of a 3-glycidoxypropyl phase, introduction of a .beta.-CD carbamate-bonded phase, and hydrolysis of the oxirane ring to a diol phase. Although the recovery of serum proteins from only a .beta.-CD bonded silica (having no diol phase) was poor, they were completely recovered from a mixed functional silica having .beta.-CD and diol phases. Direct injection analysis of drug enantiomers in serum can be attained with the mixed functional silica support over the eluent pH range of 3-7. The recovery of racemic drugs from serum was almost 100%.Keywords
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