Patterned Staining by Fluorescein-Labeled Oligonucleotides Obtained by in Vitro Selection
- 1 January 1996
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 68 (24) , 4309-4311
- https://doi.org/10.1021/ac960684m
Abstract
We describe a new means of surface staining using a fluorescent oligonucleotide. A folic acid-specific, fluorescent-labeled oligonucleotide was selected from a group of randomly sequenced oligonucleotides by repeated elution through a folic acid-immobilized column and amplified by the polymerase chain reaction. We then examined the oligonucleotide scattered on a polyester film with folic acid immobilized in a defined pattern, by means of laser fluorescence microscopy. The results showed that the selected oligonucleotide fluoresced exactly following the pattern of immobilized folic acid.Keywords
This publication has 13 references indexed in Scilit:
- A Biosensor Array Based on PolyanilineAnalytical Chemistry, 1996
- Spatially-Addressable Immobilization of Macromolecules on Solid SupportsJournal of the American Chemical Society, 1995
- Structural Changes of Bovine Serum Albumin upon Adsorption to Modified Fluoropolymer Substrates Used for Neural Cell Attachment StudiesLangmuir, 1995
- Micron‐Scale Patterning of Biological MoleculesAngewandte Chemie International Edition in English, 1995
- Novel Surface Graft Copolymerization Method with Micron-Order Regional PrecisionMacromolecules, 1994
- Deuterium isotope effects in constrained tryptophan derivatives: implications for tryptophan photophysicsJournal of the American Chemical Society, 1992
- Micropatterning Proteins and Synthetic Peptides on Solid Supports: A Novel Application for Microelectronics Fabrication TechnologyBiotechnology Progress, 1992
- Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structuresNature, 1992
- In vitro selection of RNA molecules that bind specific ligandsNature, 1990
- DNA typing from single hairsNature, 1988