Large scale isolation of nuclei and nucleoli from vitellogenic oocytes of Xenopus laevis
- 1 January 1978
- journal article
- research article
- Published by Springer Nature in Chromosoma
- Vol. 66 (4) , 299-308
- https://doi.org/10.1007/bf00328531
Abstract
A new method is described which allows the purification of large quantities of nuclei (germinal vesicles) from Xenopus laevis vitellogenic oocytes of different developmental stages. From the isolated nuclei, purified nucleoli were obtained. The isolated germinal vesicles are transcriptionally active showing endogenous RNA polymerase activity as well as a high level of activity with an exogenously added template.Keywords
This publication has 15 references indexed in Scilit:
- Transcription of Xenopus tDNAFormula and Sea Urchin Histone DNA Injected into the Xenopus Oocyte NucleusPublished by Cold Spring Harbor Laboratory ,1978
- Coupled transcription-translation of DNA injected into Xenopus oocytesCell, 1977
- Isolation of ribosomal gene chromatinDevelopmental Biology, 1977
- Defined nutrient medium for the in vitro maintenance ofXenopus laevis oocytesIn Vitro Cellular & Developmental Biology - Plant, 1976
- Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animalsJournal of Morphology, 1972
- Recherches biochimiques sur l'oogene`seDevelopmental Biology, 1971
- Localization of amplified ribosomal DNA in the oocyte of Xenopus laevisBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968
- Specific Gene Amplification in OocytesScience, 1968
- Persistence in early amphibian embryos of informational RNA's from the lampbrush chromosome stage of oögenesis.Proceedings of the National Academy of Sciences, 1967
- CHROMOSOMAL PHYSIOLOGY IN RELATION TO NUCLEAR STRUCTUREAnnals of the New York Academy of Sciences, 1950