Expression of the alpha subunit of PABA peptide hydrolase (EC 3.4.24.18) in MDCK cells
- 13 December 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 335 (3) , 376-379
- https://doi.org/10.1016/0014-5793(93)80422-q
Abstract
In this paper, we report the expression of PPHα in the polarized cell line MDCK (Madin Darby canine kidney). In these cells, the enzyme was synthesized m an inactive profonn, which upon treatment with trypsin was activated. The enzyme isolated from cell extracts was core-glycosylated and appeared to be retained in the ER as a homodimer. No PPHα was detectable on the surface of intact cells by immunofluoreseence. However, a complex glycosylated soluble but inactive form was present in the culture medium, suggesting that proteolytic removal of the C-terminal membrane anchoring peptide leads to the secretion of PPHα.Keywords
This publication has 26 references indexed in Scilit:
- Cloning of the PABA peptide hydrolase alpha subunit (PPHα) from human small intestine and its expression in COS‐1 cellsFEBS Letters, 1993
- Implications of the three‐dimensional structure of astacin for the structure and function of the astacin family of zinc‐endopeptidasesEuropean Journal of Biochemistry, 1993
- Refined 1·8 Å X-ray Crystal Structure of Astacin, a Zinc-endopeptidase from the Crayfish Astacus astacus L.: Structure Determination, Refinement, Molecular Structure and Comparison with ThermolysinJournal of Molecular Biology, 1993
- Proteolytic processing of human intestinal lactase‐phlorizin hydrolase precursor is not a prerequisite for correct sorting in Madin Darby canine kidney (MDCK) cellsFEBS Letters, 1992
- Maturation of human lactase‐phlorizin hydrolase Proteolytic cleavage of precursor occurs after passage through the Golgi complexFEBS Letters, 1992
- Isolation of cDNAs for LCE and HCE, two constituent proteases of the hatching enzyme of Oryzias latipes, and concurrent expression of their mRNAs during developmentDevelopmental Biology, 1992
- Molecular cloning of the α‐subunit of rat endopeptidase‐24.18 (endopeptidase‐2) and co‐localization with endopeptidase‐24.11 in rat kidney by in situ hybridizationFEBS Letters, 1992
- The Drosophila dorsal-ventral patterning gene tolloid is related to human bone morphogenetic protein 1Cell, 1991
- Novel Regulators of Bone Formation: Molecular Clones and ActivitiesScience, 1988
- Expression and intracellular transport of microvillus membrane hydrolases in human intestinal epithelial cells.The Journal of cell biology, 1985