Optimization of Streptomyces aureofaciens transformation and disruption of the hrdA gene encoding a homologue of the principal factor
Open Access
- 1 October 1993
- journal article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 139 (10) , 2525-2529
- https://doi.org/10.1099/00221287-139-10-2525
Abstract
SUMMARY: The metabolism of 2:4-dichlorophenoxyacetic acid and 4-chloro-2-methylphenoxyacetic acid by a strain of Flavobacterium peregrinum and an Achromobacter sp., respectively, has been studied. Bacteria from young cultures were more active than those from older ones in oxidizing these substrates. Evidence is presented that adapted organisms dissimilate 2:4-dichlorophenoxyacetic acid through 2:4-dichlorophenol and 4-chlorocatechol, and that 4-chloro-2-methylphenoxyacetic acid is dissimilated through 5-chloro-2-cresol. Bacteria grown on 2:4-dichlorophenoxyacetic acid do not oxidize any of the other five possible isomers, but can oxidize 2:4-dibromo-, 4-bromo-2-chloro-, 4-chloro-, and, to a small extent, 2-chlorophenoxyacetic acids.Keywords
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