An Improved Method for the Purification of “Light” Noradrenaline Vesicles from Rat Vas Deferens: Some Biochemical Characteristics
- 1 February 1983
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 41 (2) , 466-472
- https://doi.org/10.1111/j.1471-4159.1983.tb04764.x
Abstract
Light noradrenaline [norepinephrine] storage vesicles from nerve endings were isolated by differential centrifugation and differential gradient centrifugation. They were further purified by isopycnic sucrose/D2O centrifugation. By using these centrifugation techniques, an isopycnic gradient fraction was observed in which noradrenaline was enriched .apprx. 41 times vs. a total homogenate. This factor could be raised to 61 by using seminal ducts of castrated rats. Comparison of the distribution patterns in sucrose/D2O isopycnic gradients indicated that light noradrenaline vesicles of nerve endings contain Mg2+-stimulated ATPase and ATP, but that only a minor part of the dopamine .beta.-hydroxylase can be associated with these vesicles.Keywords
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