De novo synthesis of a polymer of deoxyadenylate and deoxythymidylate by calf thymus DNA polymerase alpha.
- 1 October 1975
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (10) , 3944-3946
- https://doi.org/10.1073/pnas.72.10.3944
Abstract
In a reaction mixture containing calf thymus DNA polymerase alpha (DNA nucleotidyltransferase; deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase; EC 2.7.7.7), calf thymus DNA unwinding protein, DNA, deoxyadenosine 5'-triphosphate and deoxythymidine 5'-triphosphate, a copolymer of deoxyadenylate and deoxythymidylate is synthesized after a lag period of 1-2 hr. In the presence of the four deoxyribonucleoside triphosphates only deoxyadenylate and deoxythymidylate are incorporated into the polymer and the rate of synthesis is decreased. The reaction variably occurs in the absence of DNA or DNA unwinding protein but with a greatly entended lag period. The optimal Mg2+ concentration for synthesis of the polymer of deoxyadenylate and deoxythymidylate is 1 mM, in contrast to an optimal Mg2+ concentration of 8 mM for DNA synthesis with activated DNA as template. Characterization of the product of de novo synthesis indicates that it is the alternating copolymer, poly(dA-dT).Keywords
This publication has 18 references indexed in Scilit:
- Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions.1972
- Base Sequence and Evolution of Guinea-pig α-Satellite DNANature, 1970
- Comparison of the requirements for ribonucleic acid synthesis with the requirements for deoxyribonucleic acid synthesis in animal tissuesJournal of Cellular Physiology, 1967
- Sedimentation studies of the size and shape of DNAJournal of Molecular Biology, 1965
- DEOXYNUCLEOTIDE-POLYMERIZING ENZYMES OF CALF THYMUS GLAND .I. LARGE SCALE PURIFICATION OF TERMINAL AND REPLICATIVE DEOXYNUCLOEOTIDYL TRANSFERASES1965
- ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID .11. FURTHER STUDIES ON NEAREST NEIGHBOR BASE SEQUENCES IN DEOXYRIBONUCLEIC ACIDS1962
- ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID .12. POLYMER OF DEOXYGUANYLATE AND DEOXYCYTIDYLATE1962
- ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID .9. POLYMERASE FORMED AFTER T2 BACTERIOPHAGE INFECTION OF ESCHERICHIA COLI - NEW ENZYME1962
- ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID .8. FREQUENCIES OF NEAREST NEIGHBOR BASE SEQUENCES IN DEOXYRIBONUCLEIC ACID1961
- Enzymatic synthesis of deoxyribonucleic acid. VII. Synthesis of a polymer of deoxyadenylate and deoxythymidylate.1960