Monoclonal Antibody to Blood Group Glycosyltransferases, Produced by Hybrids Constructed with Epstein-Barr-Virus-Transformed B Lymphocytes from a Patient with ABO-Incompatible Bone Marrow Transplant and Mouse Myeloma Cells
- 31 July 1990
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 59 (2) , 116-118
- https://doi.org/10.1111/j.1423-0410.1990.tb05022.x
Abstract
A patient with chronic myeloid leukemia secreted an antibody to blood group glycosyltransferases after ABO-incompatible bone marrow transplatation (B recipient/O donor). Peripheral B lymphocytes from the recipient were transformed with Epstein-Barr virus, and then fused by polyethylene glycol with mouse myeloma cell line P3-X63/Ag8.653. After the cloning of the hybridoma cells, a cell line which produced human IgM antibody to blood group glycosyltransferases was established. The antibody completely neutralized B transferase activity at low concentration, while a larger amount of immunoglobulins was required to neutralize A transfer activity.Keywords
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