Nuclear protein phosphatases with Kelch-repeat domains modulate the response to brassinosteroids in Arabidopsis
Open Access
- 15 February 2004
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 18 (4) , 448-460
- https://doi.org/10.1101/gad.1174204
Abstract
Perception of the plant steroid hormone brassinolide (BL) by the membrane-associated receptor kinase BRI1 triggers the dephosphorylation and accumulation in the nucleus of the transcriptional modulators BES1 and BZR1. We identified bsu1-1D as a dominant suppressor of bri1 in Arabidopsis. BSU1 encodes a nuclear-localized serine–threonine protein phosphatase with an N-terminal Kelch-repeat domain, and is preferentially expressed in elongating cells. BSU1 is able to modulate the phosphorylation state of BES1, counteracting the action of the glycogen synthase kinase-3 BIN2, and leading to increased steady-state levels of dephosphorylated BES1. BSU1 belongs to a small gene family; loss-of-function analyses unravel the extent of functional overlap among members of the family and confirm the role of these phosphatases in the control of cell elongation by BL. Our data indicate that BES1 is subject to antagonistic phosphorylation and dephosphorylation reactions in the nucleus, which fine-tune the amplitude of the response to BL.Keywords
This publication has 64 references indexed in Scilit:
- Genome-Wide Insertional Mutagenesis of Arabidopsis thalianaScience, 2003
- Binding of the Concave Surface of the Sds22 Superhelix to the α4/α5/α6-Triangle of Protein Phosphatase-1Journal of Biological Chemistry, 2002
- Arabidopsis Brassinosteroid-Insensitivedwarf12Mutants Are Semidominant and Defective in a Glycogen Synthase Kinase 3β-Like KinasePlant Physiology, 2002
- BES1 Accumulates in the Nucleus in Response to Brassinosteroids to Regulate Gene Expression and Promote Stem ElongationPublished by Elsevier ,2002
- Fusion genetic analysis of gibberellin signaling mutantsThe Plant Journal, 2000
- Control of β-Catenin StabilityMolecular Cell, 2000
- Conversion of Protein Phosphatase 1 Catalytic Subunit to a Mn2+-Dependent Enzyme Impairs Its Regulation by Inhibitor 1Biochemistry, 1997
- Structural basis for the recognition of regulatory subunits by the catalytic subunit of protein phosphatase 1The EMBO Journal, 1997
- Genetic evidence for an essential role of brassinosteroids in plant developmentThe Plant Journal, 1996
- Brassinosteroids Rescue the Deficiency of CYP90, a Cytochrome P450, Controlling Cell Elongation and De-etiolation in ArabidopsisPublished by Elsevier ,1996