Adsorption models of hybridization behaviour on oligonucleotide microarrays
Abstract
Analysis of data from an Affymetrix Latin Square spike-in experiment indicates that measured fluorescence intensities of features on an oligonucleotide microarray are related to spike-in RNA target concentrations via a hyperbolic response function. This function is generally identified as a Langmuir adsorption isotherm. Furthermore the asymptotic signal at high spike-in concentrations is almost invariably lower for a mismatch feature than for its partner perfect match feature. We survey a number of theoretical adsorption models of hybridization at the microarray surface and assess their ability to explain these observations. Of the models considered, we find the most promising model to be one of equilibrium hybridization followed by partial dissociation of duplexes during the post-hybridization washing phase.Keywords
All Related Versions
This publication has 0 references indexed in Scilit: