Characterization of Charge Isomers of Yeast Phosphoglycerate Kinase. Evidence for Intracellular Differences.
- 1 January 1976
- journal article
- research article
- Published by Danish Chemical Society in Acta Chemica Scandinavica
- Vol. 30b (3) , 228-234
- https://doi.org/10.3891/acta.chem.scand.30b-0228
Abstract
Three electrophoretic components of phosphoglycerate kinase (EC 2.7.2.3) were isolated from baker''s yeast. The isoionic point of the major component is 7.18 at 10.degree. C. Corresponding values for the minor ones are 6.91 and 7.48, respectively. There is a difference of 1 charge-unit between the isomers 1 and 2, and between the isomers 2 and 3. The release of component 3 from the yeast cells appears in contrast to the isomers 1 and 2 to be promoted by an organic solvent, thus suggesting this component to be bound to the cell-membrane. The amino-terminal amino acid residue appears to be N-acetylated serine in each of the 3 cases. The carboxyl-terminal ends seem to be identical with -(Ala, Leu, Val, Lys)- Ala-Lys as the ultimate sequence. From circular dichroism spectra the contents of .alpha.-helix and .beta.-structure were estimated to 15 and 40-50%, respectively. Factors were determined for transformation and comparison of the specific activities as determined under the various conditions used at different laboratories.This publication has 7 references indexed in Scilit:
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