Ectopic Expression of BABY BOOM Triggers a Conversion from Vegetative to Embryonic Growth
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Open Access
- 18 July 2002
- journal article
- Published by Oxford University Press (OUP) in Plant Cell
- Vol. 14 (8) , 1737-1749
- https://doi.org/10.1105/tpc.001941
Abstract
The molecular mechanisms underlying the initiation and maintenance of the embryonic pathway in plants are largely unknown. To obtain more insight into these processes, we used subtractive hybridization to identify genes that are upregulated during the in vitro induction of embryo development from immature pollen grains of Brassica napus (microspore embryogenesis). One of the genes identified, BABY BOOM (BBM), shows similarity to the AP2/ERF family of transcription factors and is expressed preferentially in developing embryos and seeds. Ectopic expression of BBM in Arabidopsis and Brassica led to the spontaneous formation of somatic embryos and cotyledon-like structures on seedlings. Ectopic BBM expression induced additional pleiotropic phenotypes, including neoplastic growth, hormone-free regeneration of explants, and alterations in leaf and flower morphology. The expression pattern of BBM in developing seeds combined with the BBM overexpression phenotype suggests a role for this gene in promoting cell proliferation and morphogenesis during embryogenesis.Keywords
This publication has 51 references indexed in Scilit:
- Ectopic expression ofAINTEGUMENTA inArabidopsis plants results in increased growth of floral organsDevelopmental Genetics, 1999
- Fundamental Concepts in the Embryogenesis of Dicotyledons: A Morphological Interpretation of Embryo Mutants.Plant Cell, 1997
- Photo and hormonal control of meristem identity in the Arabidopsis flower mutants apetala2 and apetala1.Plant Cell, 1997
- A Dissociation insertion causes a semidominant mutation that increases expression of TINY, an Arabidopsis gene related to APETALA2.Plant Cell, 1996
- The AINTEGUMENTA gene of Arabidopsis required for ovule and female gametophyte development is related to the floral homeotic gene APETALA2.Plant Cell, 1996
- pBINPLUS: An improved plant transformation vector based on pBIN19Transgenic Research, 1995
- The APETALA2 domain is related to a novel type of DNA binding domain.Plant Cell, 1995
- LEAFY COTYLEDON1 Is an Essential Regulator of Late Embryogenesis and Cotyledon Identity in Arabidopsis.Plant Cell, 1994
- Leafy Cotyledon Mutants of Arabidopsis.Plant Cell, 1994
- Identification of Embryoid-Abundant Genes That Are Temporally Expressed during Pollen Embryogenesis in Wheat Anther CulturesPlant Physiology, 1992