INVITRO PRODUCTION AND CELLULAR-ORIGIN OF MURINE TYPE-II INTERFERON

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 36  (4) , 883-890
Abstract
Antigen[Ag]-specific type II interferon was produced in vitro by harvesting supernatants of spleen cell cultures from Swiss-Webster mice sensitized with Mycobacterium bovis strain BCG and challenged with old tuberculin. Treatment of C3H mouse spleen cell cultures with appropriate anti-Ia [immune response-associated Ag], anti-IgG, anti-Thy-1 or anti-Ly-2,3 sera resulted in a significant decrease in production of type II interferon. Removal of nylon wool adherent cells or cells with histamine receptors by column chromatography similarly caused reduced production of type II interferon. Recombination of spleen cell cultures treated with anti-Ia and anti-Thy-1 sera or of cells treated with anti-IgG and anti-Thy-1 resulted in restored production of type II interferon. Interferon production was restored by combination of cells passed through histamine columns with anti-Ia treated cells or those passed through nylon wool columns with anti-Thy-1 treated cells. Anti-Ly-1 serum treatment had no effect on interferon production. Removal of plastic-adherent cells or cells that had phagocytosed carbonyl iron decreased interferon production, suggesting that macrophages were involved in type II interferon production. Recombination of non-adherent spleen cells with anti-Ia and anti-Thy-1 sera treated spleen cells, did not restore interferon production, suggesting macrophages and other cells are depleted by the adherence procedure. Type II interferon may be produced by suppressor or cytotoxic (Ly-2,3+) T [thymus-derived] lymphocytes in cooperation with 1 or 2 additional cell types: B [bone marrow-derived] lymphocytes and macrophages.

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