Complete Separation of Albumin and Hemoglobin by Metal Chelate Affinity Chromatography
- 1 May 1991
- journal article
- research article
- Published by Taylor & Francis in Separation Science and Technology
- Vol. 26 (5) , 637-645
- https://doi.org/10.1080/01496399108049905
Abstract
Hemoglobin was adsorbed on Zn2+ chelating Sepharose 6B but albumin was not adsorbed. Then albumin and hemoglobin could be separated completely by metal chelate affinity chromatography. In a conventional columnwise adsorption step, unusual breakthrough curves were observed because some parts of metal ions could be released from the adsorbent. A combined process with batchwise adsorption and columnwise elution was used to avoid this problem and to shorten the operating time.Keywords
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