Application of an osmium-maceration technique to observe plant–microbe interfaces of Italian ryegrass and crown rust fungi by scanning electron microscopy

Publisher Website
Google Scholar
Abstract
The osmium-maceration technique (osmium–DMSO–osmium method) was used to observe intracellular structures of Italian ryegrass leaves infected with crown rust fungus (Puccinia coronata Corda var. coronata) by scanning electron microscopy. Prolonged maceration with dilute osmium removed the cytoplasmic matrix, and intracellular structures were disclosed in a bold relief. The three-dimensional configuration of nuclei, mitochondria, the endoplasmic reticulum, and other structures of host and parasite cells were visible. Key words: scanning electron microscopy (SEM), osmic maceration, intracellular structure, Puccinia coronata, Italian ryegrass.

This publication has 0 references indexed in Scilit: