Anguillospora longissima and A. gigantea were studied. They grew best at 19[degree]C and 28[degree]C, respectively, in non-shake culture and both at 28[degree]C in shake culture, between pH 6.5 and pH 6.7, and attain max. dry wt. in 3-4 weeks in a liquid medium composed of glucose, mineral salts and yeast extract. Both spp. were heterotrophic for the vit. thiamin and a carbohydrate source only. Both are able to utilize nitrate as a source of N and sulfate as a source of S. Both organisms can utilize starch, cellulose, cellobiose, sucrose, maltose, glucose, galactose, mannose, and xylose. A . gigantea also grows well on arabinose. Neither sp. grew well on pectin.