Abstract
Tris (hydroxyrnethyl) aminomethane was found to inhibit the growth of Haematococcus pluvialis in ASMG medium, which is a modification of ASM medium. This inhibition could be reduced by increasing the calcium concentration of the medium. Other cations, either alone or in combination with calcium, had no effect on Tris toxicity. Tolerance towards Tris was increased in the presence of 5 mM calcium when the initial pH of the medium was increased from pH 7.0 to 8.0 but pH had no effect on Tris tolerance in ASMG medium containing the normal level of calcium. These results with Haematococcus are compared with the results of a previous study on Microcystis aeruginosa.

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