Degradation of a Caulobacter Soluble Cytoplasmic Chemoreceptor Is ClpX Dependent
- 1 December 2002
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 184 (23) , 6635-6641
- https://doi.org/10.1128/jb.184.23.6635-6642.2002
Abstract
In order to determine whether ClpXP-mediated proteolysis is a common mechanism used to regulate the chemotaxis machinery during the cell cycle of Caulobacter crescentus , we have characterized a soluble cytoplasmic chemoreceptor, McpB. The mcpB gene lies adjacent to the major chemotaxis operon, which encodes 12 chemotaxis proteins, including the membrane chemoreceptor McpA. Like McpA, McpB possesses a C-terminal CheBR docking motif and three potential methylation sites, which we suggest are methylated. The McpB protein is degraded via a ClpX-dependent pathway during the swarmer-to-stalked cell transition, and a motif, which is 3 amino acids N-terminal to the McpB CheBR docking site, is required for proteolysis. Analysis of the degradation signal in McpB and McpA reveals a common motif present in the other four chemoreceptors that possess CheBR docking sites. A green fluorescent protein (GFP) fusion bearing 58 amino acids from the C terminus of McpA, which contains this motif, is degraded, suggesting that the C-terminal sequence is sufficient to confer ClpXP protease susceptibility.Keywords
This publication has 44 references indexed in Scilit:
- Proteolysis of the Caulobacter McpA Chemoreceptor Is Cell Cycle Regulated by a ClpX-Dependent PathwayJournal of Bacteriology, 2001
- Complete genome sequence of Caulobacter crescentusProceedings of the National Academy of Sciences, 2001
- Global Analysis of the Genetic Network Controlling a Bacterial Cell CycleScience, 2000
- The control of temporal and spatial organization during the Caulobacter cell cycleCurrent Opinion in Genetics & Development, 1996
- Polar Location of the Chemoreceptor Complex in the Escherichia coli CellScience, 1993
- Requirement of the Carboxyl Terminus of a Bacterial Chemoreceptor for Its Targeted ProteolysisScience, 1993
- Positional information during Caulobacter cell differentiationCurrent Opinion in Genetics & Development, 1991
- Caulobacter crescentus flagellar filament has a right-handed helical formJournal of Molecular Biology, 1984
- A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative BacteriaBio/Technology, 1983
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979