A 36‐kDa mitochondrial protein is responsible for cyanide‐resistant respiration in Hansenula anomala

7 May 1990

journal article
Published by Wiley in FEBS Letters

Vol. 264 (1) , 149-152
https://doi.org/10.1016/0014-5793(90)80787-j

Abstract

Antimycin A-dependent induction of cyanide-resistant respiration in Hansenula anomala was reversibly blocked by carbonylcyanide-m-chlorophenylhydrazone (CCCP). When the cells were pulse-labeled with [³⁵S]methionine in the presence of both antimycin A and CCCP, the radioactivity was incorporated into a 39 kDa mitochondrial protein. Upon removal of CCCP, this protein was processed into a 36 kDa form. The increase in the 36 kDa protein completely paralleled that in cyanide-resistant respiration activity, suggesting that the 39 kDa protein is the precursor of the 36 kDa protein, which is responsible for cyanide-resistant respiration.

Keywords

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