Effect of Disalicylidenepropanediamine on the Light-dependent Reduction of Carbon Dioxide and Glycerate 3-Phosphate in Intact Spinach Chloroplasts
Open Access
- 1 January 1975
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 55 (1) , 12-14
- https://doi.org/10.1104/pp.55.1.12
Abstract
Disalicylidenepropanediamine (DSPD) at 0.1 to 1 mm levels inhibited light-dependent 14CO2 assimilation in intact spinach chloroplasts about 50 to 80%, and this inhibition was accompanied by an increased ratio of 14C-glycerate 3-phosphate to 14C-glyceraldehyde 3-phosphate. Enzymatic analysis established that DSPD also inhibited the light-dependent reduction of glycerate 3-phosphate in intact spinach chloroplasts. DSPD at 0.5 mm did not inhibit ribose 5-phosphate isomerase, ribulose 5-phosphate kinase, glycerate 3-phosphate kinase, NADP+-linked glyceraldehyde 3-phosphate dehydrogenase or ribulose 1,5-diphosphate carboxylase. The inhibition of chloroplast 14CO2 assimilation by DSPD appeared to be related to the inhibition of the photosynthetic electron transport chain. These observations are consistent with experimental results which demonstrated that DSPD inhibited directly the chloroplast lamellar membrane-mediated, light-dependent reduction of ferredoxin (Trebst, A. and M. Burba, 1967, Z. Pflanzenphysiol. 57: 419-433 and Ben-Amotz, A. and M. Avron, 1972, Plant Physiol. 49: 244-248).Keywords
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