Purification of murine T cell growth factor. A lymphocyte mitogen with helper activity.

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Abstract
Mouse T cell growth factor was purified from the serum-free conditioned medium of lectin[concanavalin A]-stimulated spleen cells. A 3000-fold purification was achieved with a final yield of 12%. The purified protein, with an apparent MW of 23,000 by sodium dodecyl sulfate polyacrylamide gel electrophoresis, was active at concentrations of 4 .times. 10-11 M in the T cell growth factor and T cell replacing factor assays. Purified T cell growth factor alone was mitogenic for spleen cells from nude and normal mice.
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