Diffusion-Dependent Peak Broadening in Pore Gradient Electrophoresis

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Abstract
An earlier theory of the kinetics of pore gradient electrophoresis has been extended and generalized to include diffusion broadening of peaks. If D and D 0 represent the diffusion coefficient of a molecular species in the gel and in the absence of a gel, respectively, and M and M 0 the respective mobilities, and these variable are assumed to satisfy where x is distance, then an exact solution is obtained for the resulting model. Further, an approximate theory has been developed for the determination of diffusion broadening when diffusion coefficient and mobility are allowed to have any more general dependence on distance, provided that diffusion is a small effect. A comparison of the exact and approximate solutions shows that the error due to the approximation is usually smaller than measurement error.

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