STUDIES ON BLUETONGUE .3. COMPARISON OF 2 COMPLEMENT-FIXATION METHODS
- 1 January 1967
- journal article
- research article
- Vol. 31 (7) , 166-+
Abstract
The complement-fixation test used at Onderstepoort was compared with the method used at A.D.R.I. [Animal Diseases Research Institute] on infected calf and sheep sera. In the first method, the tests are incubated at 37[degree]C for 90 minutes and the test sera are inactivated at 53[degree]C; whereas in the A.D.R.I. method, the test sera are inactivated at 60C for 30 minutes, incubation is at 9[degree]C for 18 hours, and guinea-pig complement is supplemented with 5 per cent fresh, non-inactivated, normal calf serum. Serial serum samples from one of six experiment tally infected calves were negative in the Onderstepoort test, three calves gave only trace reactions and two showed maximum titres of 1:10 whereas all six had maximum serum titres of 1:10 to 1:80 in the A.D.R.I. test. A good correlation was obtained, however, between the results of the two methods with the sera of experimentally inoculated sheep although titres 3 to 8 times higher were obtained with the A.D.R.I.'' s test. Post inoculation bleedings from each sheep reacted in both tests.This publication has 5 references indexed in Scilit:
- STUDIES ON BLUETONGUE .4. STUDIES OF 3 STRAINS IN PRIMARY BOVINE FOETAL KIDNEY CELL CULTURES1967
- STUDIES ON BLUETONGUE .5. DETECTION OF VIRUS IN INFECTED MATERIALS BY IMMUNOFLUORESCENCE1967
- STUDIES ON BLUETONGUE .6. ANIMAL TRANSMISSION STUDIES1967
- STUDIES ON BLUETONGUE 2 - COMPLEMENT-FIXING ACTIVITY OF OVINE AND BOVINE SERA1965
- Technique of a Modified Direct Complement-Fixation Test for Viral Antibodies in Heat Inactivated Cattle Serum.1960