Synthesis of the Phytoalexin Pisatin by a Methyltransferase from Pea
- 1 January 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 80 (1) , 277-279
- https://doi.org/10.1104/pp.80.1.277
Abstract
Previous labeling studies in vivo suggest that the terminal step of (+)pisatin biosynthesis in Pisum sativum L. is methylation of the phenol (+)6a-hydroxymaackiain (HMK). We have found that extracts from pea seedlings perform this reaction, using S-adenosylmethionine as the methyl donor. The enzyme activity was induced by microbial infection or treatment with CuCl2, which elicit pisatin synthesis, though some activity was also present in healthy tissues. It has been reported that CuCl2-treated pea tissue provided with (−)HMK or (−)maackiain can synthesize (−)pisatin. Our extract showed no methyltransferase activity dependent on either of these substrates. Methylation of (+)maackiain was detectable, but much slower than that of (+)HMK.This publication has 14 references indexed in Scilit:
- Elicitor rapidly induces chalcone synthase mRNA in Phaseolus vulgaris cells at the onset of the phytoalexin defense responseProceedings of the National Academy of Sciences, 1984
- Phytoalexin synthesis in soybean cells: Elicitor induction of phenylalanine ammonia-lyase and chalcone synthase mRNAs and correlation with phytoalexin accumulationArchives of Biochemistry and Biophysics, 1984
- Induction of phytoalexin synthesis in soybean. Stereospecific 3,9‐dihydroxypterocarpan 6a‐hydroxylase from elicitor‐induced soybean cell culturesEuropean Journal of Biochemistry, 1984
- Enzyme induction in soybean infected by Phytophthora megasperma f.sp. glycineaArchives of Biochemistry and Biophysics, 1982
- Metabolism of the phytoalexins medicarpin and maackiain by Fusarium solaniPhytochemistry, 1982
- Induction of enzymes of phytoalexin synthesis in cultured soybean cells by an elicitor from Phytophthora megasperma f. sp. glycineaPlant Cell Reports, 1982
- Effects of Light and of Fusarium solani on Synthesis and Activity of Phenylalanine Ammonia-Lyase in PeasPlant Physiology, 1981
- The association of pisatin tolerance and demethylation with virulence on pea in Nectria haematococcaPhysiological Plant Pathology, 1980
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- The Relation Between Pisatin and the Development of Aphanomyces euteiches in Diseased Pisum sativumPhytopathology®, 1976