Monoclonal antibodies that recognize discrete forms of tubulin.
- 1 April 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (8) , 2579-2583
- https://doi.org/10.1073/pnas.79.8.2579
Abstract
Anti-tubulin antibodies secreted by mouse plasmacytoma NSI-spleen cell hybrids were detected by an indirect binding assay. Different antibodies bound to different combinations of the rat brain tubulins as resolved by isoelectric focusing. Two monoclonal antibodies (TUB 2.1 and TUB 2.5) labeled only the tubulin band on a polyacrylamide electropherogram and .beta.-tubulins as resolved by isoelectric focusing. The fraction that was specifically bound and eluted from antibody affinity columns was enriched in .beta.-tubulins compared with .alpha.-tubulins, suggesting the possibility of some soluble tubulin homodimers and .alpha.,.beta.-heterodimers. Double labeling experiments were used to show that all detectable microtubules contained .beta.-tubulin.This publication has 30 references indexed in Scilit:
- Monoclonal antibodies which recognize different cell types in the rat retinaNature, 1980
- Number and evolutionary conservation of α- and β-tubulin and cytoplasmic β- and γ-actin genes using specific cloned cDNA probesCell, 1980
- Translation in vitro of Rat Brain mRNA Coding for a Variety of Tubulin FormsEuropean Journal of Biochemistry, 1980
- Changes in tubulin heterogeneity during postnatal development of rat brainBiochemical and Biophysical Research Communications, 1979
- CHARACTERIZATION OF MULTIPLE FORMS OF BRAIN TUBULIN SUBUNITSJournal of Neurochemistry, 1978
- Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysisCell, 1978
- Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized filmFEBS Letters, 1977
- Cellular Distribution, Purification, and Molecular Nature of Human la AntigensScandinavian Journal of Immunology, 1977
- Antibodies to major histocompatibility antigens produced by hybrid cell linesNature, 1977
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970