A combination of sister chromatid differential staining and giemsa banding
- 1 August 1975
- journal article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 31 (8) , 916-918
- https://doi.org/10.1007/bf02358850
Abstract
We report a procedure for combining sister chromatid differential staining and G banding in the same metaphase plate. Mammalian cells in culture are grown in medium containing 5-bromodeoxyuridine for two cell cycles, and conventional air-dried preparations are made. The slides are treated with a trypsin or a urea solution the same way as for regular G banding. This method is simple and fast and provides additional information for cytogeneticists.Keywords
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