Gene Transfer and Expression Studies in Cultured Avian Neural Crest Cells Differentiating Into Melanocytes
- 1 January 1989
- journal article
- research article
- Published by Wiley in Pigment Cell Research
- Vol. 2 (1) , 44-52
- https://doi.org/10.1111/j.1600-0749.1989.tb00157.x
Abstract
Neural crest cells obtained from explanted neural tubes take up express, and retain exogenous DNA applied by the CaPO4 co-precipitation method during their differnetiation into melanocytes. High efficiencies of gene transfer were obtained with both supercoiled DNA and intact phage particles; linear DNA or DNA from the phage yielded very low efficiencies. There is some evidence that transferred gene expression is differentiation dependent. The system should be useful for studies concerned with the analysis of cell developmental genes and their regulatory elements.Keywords
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