Heregulin β1 induces the down regulation and the ubiquitin‐proteasome degradation pathway of p185HER2 oncoprotein
Open Access
- 30 January 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 422 (2) , 129-131
- https://doi.org/10.1016/s0014-5793(97)01612-8
Abstract
Analysis of the fate of the p185HER2 oncoprotein following activation by heregulin β1 revealed the induction of the tyrosine‐phosphorylation, down‐modulation, and polyubiquitination of p185HER2. Receptor ubiquitination was suppressed in cells treated with heregulin β1 in the presence of sodium azide, an inhibitor of ATP‐dependent reactions, or genistein, a tyrosine kinase protein inhibitor, indicating the requirement for kinase activity and ATP in p185HER2 polyubiquitination. Ubiquitinated p185HER2 was degradated by the 26S proteasome proteolytic pathway. Kinetics and inhibition experiments indicated that endocytosis of the receptor occurs downstream of the initiation of the degradation process.Keywords
This publication has 19 references indexed in Scilit:
- T Cell Antigen Receptor Ubiquitination Is a Consequence of Receptor-mediated Tyrosine Kinase ActivationPublished by Elsevier ,1996
- All ErbB Receptors Other Than the Epidermal Growth Factor Receptor Are Endocytosis ImpairedJournal of Biological Chemistry, 1996
- Degradation Process of Ligand-stimulated Platelet-derived Growth Factor β -Receptor Involves Ubiquitin-Proteasome Proteolytic PathwayPublished by Elsevier ,1995
- Herbimycin A Induces the 20 S Proteasome- and Ubiquitin-dependent Degradation of Receptor Tyrosine KinasesJournal of Biological Chemistry, 1995
- Roles for a Cytoplasmic Tyrosine and Tyrosine Kinase Activity in the Interactions of Neu Receptors with Coated PitsJournal of Biological Chemistry, 1995
- A neu acquaintance for ErbB3 and ErbB4: A role for receptor heterodimerization in growth signalingCell, 1994
- Endocytosis of growth factor receptorsBioEssays, 1993
- p185 HER2/neu Epitope Mapping with Murine Monoclonal AntibodiesHybridoma, 1992
- Neu differentiation factor: A transmembrane glycoprotein containing an EGF domain and an immunoglobulin homology unitCell, 1992
- Establishment of a European registry for familial ovarian cancerEuropean Journal of Cancer and Clinical Oncology, 1991