A Double-Dish Method of Cultivating Fungi

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Abstract
In this double-dish method the outer dish is a 9 cm Petri plate containing the nutrient agar. The inner dish, consisting of a cel-lose membrane base attached to a lucite ring, is placed on this agar surface. The fungus, Trichophyton mentagrophytes, is inoculated directly onto the cellulose membrane. Growth of the inoculum proceeds normal-ly, demonstrating that sufficient nutrient and water diffuse from the agar through the membrane. The fungus does not attack the membrane nor do any hyphae penetrate it. The method is useful in that it allows both the development of colony characters found in agar cultures and ease of separation of the fungus from the nutrient source.

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