Eleven tungsten atom cluster labels: high-resolution, site-specific probes for electron microscopy.
Open Access
- 1 December 1990
- journal article
- abstracts
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 38 (12) , 1787-1793
- https://doi.org/10.1177/38.12.1701458
Abstract
Two derivatives of a tungstate cluster containing 11 tungsten atoms (W11PO39SiR4-) have been synthesized which enable them to be covalently attached to biomolecules at specific sites. The tungstate cluster is 1.0 nm in diameter, electron dense, and visible in the electron microscope. One derivative is a W11-sulfonyl chloride, reactive with amines and sulfhydryls. The second compound is a W11-thiosulfonate which can be used to label sulfhydryl groups. These new labels are beam resistant and provide significantly higher resolution then most other electron microscopy (EM) markers. Labeling of the protein albumin is described as an example.Keywords
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