THE EIGHTH COMPONENT OF HUMAN COMPLEMENT (C8): ISOLATION, CHARACTERIZATION, AND HEMOLYTIC EFFICIENCY
Open Access
- 1 November 1969
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 130 (5) , 1145-1160
- https://doi.org/10.1084/jem.130.5.1145
Abstract
A method was described allowing the isolation of the eighth component of complement, C8, from human serum. C8 is an immunochemically definable, heretofore unrecognized serum protein with a molecular weight of approximately 150,000 and an electrophoretic mobility of a γ1-globulin. It may be obtained with 5% yield as a 5000-fold purified, physicochemically and immunochemically homogeneous protein. Under optimal experimental conditions, a few molecules of C8 were found sufficient for lysis of a cell.Keywords
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