Identification and purification of Namalwa nuclear RNP antigen 52/5.3
- 1 January 1983
- journal article
- research article
- Published by Springer Nature in Zeitschrift für Krebsforschung und Klinische Onkologie
- Vol. 105 (1) , 67-75
- https://doi.org/10.1007/bf00391834
Abstract
Using affinity purified rabbit antibodies to HeLa nucleoli and the Western blotting techniques, an antigen with an approximate molecular weight of 52,000 and pI of 5.3 was found in Namalwa cells (a Burkitt lymphoma), but not in normal liver cells. This antigen was purified from Namalwa RNP by column chromatography on Sephacryl S-200, hydroxylapatite and one-dimensional SDS gel electrophoresis. A liver protein with the same molecular weight and pI value was purified from RNP fraction by one-dimensional SDS gel eletrophoresis. Both proteins had similar amino-acid compositions. The tryptic map of 125I-labeled protein 52/5.3 contained approximately nine major spots; spot 9 was present in the Namalwa protein but not in the liver protein. The similarity of the structures of these proteins and their differences in antigenicity are noteworthy and require further structural and functional analysis.This publication has 36 references indexed in Scilit:
- An enzyme immunoassay for the detection of human tumor nucleolar antigensCancer Letters, 1981
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Nucleolar immunofluorescence in human hematological malignanciesLife Sciences, 1979
- Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphenylglycolurilBiochemical and Biophysical Research Communications, 1978
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Purification of anti-histone-H1 antibodies and their use in measuring histone determinants in chromatin by radioimmunoadsorbanceBiochemical and Biophysical Research Communications, 1976
- Isolation of an electrophoretically homogeneous nonhistone nucleolar proteinLife Sciences, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- The isolation and properties of deoxyribonucleoprotein particles containing single nucleic acid moleculesJournal of Molecular Biology, 1959