Biochemical Studies of Pigments from the Pathogenic Fungus, Microsporum cookei

Abstract
Some effects of a naphthoquinone pigment, xanthomegnin, isolated from Microsporum cookei on rat liver mitochondria were examined. 1. Polarographic monitoring of O2 consumption indicated that xanthomegnin is a strong uncoupler of oxidative phosphorylation in rat liver mitochondria. 2. The latent adenosinetriphosphatase [ATPase, EC 3.6.1.3] activity of freshly prepared mitochondria was markedly stimulated by xanthomegnin; this stimulation was prevented by bovine serum albumin (BSA). 3. A large-amplitude swelling of isolated mitochondria was induced by xanthomegnin in isotonic KCl; addition of BSA suppressed this induction of mitochondrial swelling.

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