Plant regeneration from protoplasts of Dimorphotheca and Rudbeckia
- 1 December 1987
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 6 (6) , 414-416
- https://doi.org/10.1007/bf00272771
Abstract
Protoplasts were isolated from leaves, shoots, cotyledons, ray florets and callus cultures of Dimorphotheca aurantiaca (syn. D. sinuata) (Cape Marigold, Star of the Veldt) and Rudbeckia hirta, R. laciniata and R. purpurea; species of ornamental value. For Dimorphotheca, plants were regenerated from protoplasts of all sources apart from the ray floret, whilst for the Rudbeckia species, although protoplast division was induced in most cases, only leaf mesophyll protoplasts of R. hirta c.v. Marmalade gave plants. The establishment of plant regeneration for these ornamental species, from protoplasts, now provides a basis for their somatic hybridisation.Keywords
This publication has 9 references indexed in Scilit:
- Plant regeneration from protoplasts of a wild lettuce species (Lactuca saligna L.)Plant Cell Reports, 1987
- Isolation, culture and callus regeneration of protoplasts of wild and cultivated Helianthus speciesPlant Cell Reports, 1986
- Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant speciesPlant Cell Reports, 1983
- The Isolation and Culture of Leaf Protoplasts of Cichorium intybus and Their Regeneration into PlantsZeitschrift für Pflanzenphysiologie, 1982
- Comparative Studies on Protoplast Regeneration in Herbaceous Species of the Dicotyledoneae ClassZeitschrift für Pflanzenphysiologie, 1981
- Chromosomal behaviour in somatic hybrids of soybean-Nicotiana glaucaMolecular Genetics and Genomics, 1977
- Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid mediaPlanta, 1975
- The isolation, culture and regeneration of Petunia leaf protoplastsDevelopmental Biology, 1973
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962