Evaluation of monoclonal antibody-based capture enzyme immunoassays for detection of specific antibodies to measles virus
- 1 July 1991
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 29 (7) , 1466-1471
- https://doi.org/10.1128/jcm.29.7.1466-1471.1991
Abstract
Monoclonal antibodies to the hemagglutinin protein, fusion protein, phosphoprotein, matrix protein, and nucleoprotein of measles virus were evaluated as detector antibodies in capture enzyme immunoassays (EIAs) for the detection of specific serum immunoglobulin G (IgG), IgA, and IgM antibodies to measles virus. A pool of monoclonal antibodies to hemagglutinin protein and nucleoprotein proved optimal and was further evaluated. Specific IgM was detected in 97% of adolescents with clinical measles, 97% of infants 3 weeks postvaccination, and less than 1% of normal serum specimens. Specific IgA antibodies were found in 97% of adolescents with clinical measles, 97% of infants 3 weeks postvaccination, and less than 1% of normal serum specimens. Specific IgA antibodies were found in 97% of clinical measles cases and vaccinees, in 26% of healthy persons, and in 36% of infants 8 months postvaccination; consequently, IgA antibodies were not a useful indicator of recent measles infection. A significant increase in IgG antibodies between paired specimens was detected in 92% of clinical cases and all vaccinees. Only 59% of infant specimens had persistent IgG antibodies as detected by capture EIA at 8 months postvaccination, whereas all specimens had antibodies as detected by hemagglutination inhibition and plaque neutralization. An alternative indirect EIA, in which antigen was directly absorbed to the solid phase, was more sensitive than the capture design, detecting IgG antibodies in all infants postvaccination. When standardized with a microneutralization assay for the detection of persistent antibodies, the indirect IgG EIA gave predictive values for positive and negative tests exceeding 90%. Our capture IgM and indirect IgG EIAs provide a practical combination of serologic tests for the determination of acute measles virus infection and past exposure to measles virus or vaccine, respectively.Keywords
This publication has 26 references indexed in Scilit:
- The Plaque-Neutralization Test as a Measure of Prior Exposure to Measles VirusThe Journal of Infectious Diseases, 1987
- Long-term antibody response after measles vaccination in an isolated arctic society in GreenlandVaccine, 1986
- Evaluation of the structural proteins of the hamster neurotropic strain of measles virus with monoclonal antibodiesJournal of Neuroimmunology, 1986
- Detection of IgG antibodies to measles virus using monoclonal antibodies for antigen-capture in enzyme immunoassayJournal of Virological Methods, 1984
- Rapid diagnosis of measles using enzyme-linked immunosorbent assay for measles immunoglobulin MDiagnostic Microbiology and Infectious Disease, 1983
- Reported measles in persons immunologically primed by prior vaccinationThe Journal of Pediatrics, 1982
- DETECTION OF MEASLES IgM ANTIBODIES BY ENZYME‐LINKED IMMUNOSORBENT ASSAY (ELISA)Acta Pathologica Microbiologica Scandinavica Series B: Microbiology, 1982
- A Solid-Phase Radioimmunoassay for IgG and IgM Antibodies Against Measles VirusJournal of General Virology, 1977
- Measles IgM response during reinfection of previously vaccinated childrenThe Journal of Pediatrics, 1973
- Measles Immunoglobulins in Subacute Sclerosing PanencephalitisBMJ, 1971