PCR‐based detection of resistance to acetyl‐CoA carboxylase‐inhibiting herbicides in black‐grass (Alopecurus myosuroides Huds) and ryegrass (Lolium rigidum Gaud)

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Abstract
A simple method based upon allele‐specific PCR was developed to detect an isoleucine‐leucine substitution in the gene encoding chloroplastic acetyl‐coenzyme A carboxylase (ACCase) in two gramineous weeds: Lolium rigidum Gaud and Alopecurus myosuroides Huds. Analysis of 1800 A myosuroides and 750 L rigidum seedlings showed that the presence of ACCase leucine allele(s) conferred cross‐resistance to the cyclohexanedione herbicide cycloxydim and to the aryloxyphenoxypropionate herbicides fenoxaprop‐P‐ethyl and diclofop‐methyl. Seedlings containing ACCase leucine allele(s) could be either sensitive or resistant to the aryloxyphenoxypropionate herbicides haloxyfop‐P‐methyl and clodinafop‐propargyl. Successful detection of resistant plants in a field population of A myosuroides was achieved using this PCR assay. Using it with basic molecular biology laboratory equipment, the presence of resistant leucine ACCase allele(s) can be detected within one working day. © 2002 Society of Chemical Industry
Funding Information
  • Conseil Régional de Bourgogne (HCP 01/5112/12)

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