Reverse Transcription-Multiplex PCR Assay for Simultaneous Detection of Escherichia coli O157:H7, Vibrio cholerae O1, and Salmonella Typhi
Open Access
- 1 November 2004
- journal article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 50 (11) , 2037-2044
- https://doi.org/10.1373/clinchem.2004.036814
Abstract
Background: Escherichia coli O157:H7, Vibrio cholerae O1, and Salmonella Typhi are pathogenic bacteria that can be found in contaminated water supplies throughout the world. No currently available assays can simultaneously detect and identify all three pathogens. Our aim was to develop a rapid and reliable technique for simultaneous detection of these pathogens.Keywords
This publication has 40 references indexed in Scilit:
- Simultaneous Detection ofEscherichia coli O157:H7,Salmonella, andShigella in Apple Cider and Produce by a Multiplex PCRJournal of Food Protection, 2004
- Typhoid FeverNew England Journal of Medicine, 2002
- Detection of Salmonella typhi by polymerase chain reaction: Implications in diagnosis of typhoid feverInfection, Genetics and Evolution, 2002
- A PCR test for detecting Escherichia coli O157:H7 based on the identification of the small inserted locus (SILO157)Journal of Applied Microbiology, 2002
- Rapid and specific identification of Shiga toxin-producing Escherichia coli in faeces by multiplex PCRLetters in Applied Microbiology, 2002
- Pathogenesis and Diagnosis of Shiga Toxin-Producing Escherichia coli InfectionsClinical Microbiology Reviews, 1998
- A universal protocol for PCR detection of 13 species of foodborne pathogens in foodsJournal of Applied Microbiology, 1997
- The polymerase chain reaction: Applications for the detection of foodborne pathogensCritical Reviews in Food Science and Nutrition, 1996
- Vero cytotoxin-producing Escherichia coli O157 in beefburgers linked to an outbreak of diarrhoea, haemorrhagic colitis and haemolytic uraemic syndrome in BritainLetters in Applied Microbiology, 1994
- Effect of stress treatments on the detection of Listeria monocytogenes and enterotoxigenic Escherichia coli by the polymerase chain reactionJournal of Applied Bacteriology, 1994