CYTOTOXICITY OF PLASMA FROM PATIENTS WITH ACUTE HEPATIC-FAILURE TO ISOLATED RABBIT HEPATOCYTES

  • 1 January 1976
    • journal article
    • research article
    • Vol. 57  (3) , 348-353
Abstract
The cytoxicity of plasma from patients with various liver diseases to rabbit hepatocytes maintained in vitro was investigated using a microcytotoxicity assay system. Plasma from patients with fulminant hepatic failure and uncomplicated viral hepatitis showed significant cytotoxicity compared to controls. The cytotoxic effect of plasma from patients with fulminant hepatic failure was reduced by charcoal hemoperfusion, or heating and dialysis. The bile acids, chenodeoxycholic acid and lithocholic acid, were cytotoxic when added to control human plasma. Such toxic factors may be responsible for the delay in liver regeneration often seen in patients with fulminant hepatic failure.