B-LYMPHOCYTE DIFFERENTIATION INDUCED BY LIPOPOLYSACCHARIDE .3. SUPPRESSION OF B CELL MATURATION BY ANTI-MOUSE IMMUNOGLOBULIN ANTIBODIES

  • 1 January 1976
    • journal article
    • research article
    • Vol. 116  (6) , 1664-1668
Abstract
Lipopolysaccharide-(LPS) induced differentiation of mouse B [bone marrow-derived] lymphocytes to cells synthesizing large amounts of cytoplasmic IgM [immunoglobulin M] and IgG2 could be suppressed by antibodies to .mu.-chains. Maximal inhibition of LPS-induced differentiation was associated with increased cellular proliferation as measured by incorporation of 3H-thymidine; treatment with anti-.mu. alone over a wide doasage range did not stimulate cellular proliferation. Spleen cells from newborn mice were suppressed by concentrations of anti-.mu. several hundred-fold lower than required for adult spleen cells; the adult pattern of susceptibility to suppression was acquired by 1 wk of age. No significant differences in susceptibility to anti-.mu. were found in comparisons of adult spleen, lymph node, bone marrow, and Peyer''s patch lymphocytes.

This publication has 0 references indexed in Scilit: