Evidence for 30–40S RNA as Precursor of the 60–70S RNA of Rous Sarcoma Virus

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Abstract
Rous sarcoma virus (RSV) harvested from cells at intervals of 3 minutes has the same density, sedimentation coefficient, and DNA polymerase as virus harvested at hourly intervals. The RNA of such RSV consists of a minor class of 60-70S RNA, a major class 30-40S RNA and a 4-12S class of RNA present in variable concentration. Upon incubation of virus harvested at 3-minute intervals at 40 C. in cell growth medium or Tris–saline, most of the 30-40S RNA is converted to 60-70S RNA. The electrophoretic mobility of the 30-40S RNA of RSV harvested at 3-minute intervals is lower than that of the 30-40S subunits of completely dissociated 60-70S RNA; after heating their mobilities are identical. Heating also releases some small RNA’s from 30-40S RNA of RSV harvested at 3-minute intervals, but five times more 4S RNA is released if the 30-40S RNA is allowed to convert to 60-70S in the virus. It is suggested that association of 30-40S RNA’s with some RNA’s of the 4-12S class may take place simultaneously with their conversion to 60-70S RNA.

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