Analysis of inactive renin by renin profragment monoclonal antibodies
- 20 October 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 207 (1) , 100-104
- https://doi.org/10.1016/0014-5793(86)80020-5
Abstract
Two peptides were synthesized, corresponding to the sequences (−19 to −7) and (−26 to −17) of the prorenin prosegment. Monoclonal antibodies were raised to these sequences and used to characterize human plasma inactive renin. Only anti (−19 to −7) reacted with inactive renin, as measured by direct assay or affinity chromatography. The data were used to evaluate two possible inactive renin stuctures: (i) plasma inactive renin is a truncated prorenin lacking the prosegment N-terminal portion; (ii) its spatial conformation masks the N-terminal extremity, preventing interaction of this region with specific antibodies.Keywords
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