Trägergebundene Serotoninderivate und Affinitätschromatographie synaptischer Membranproteine / Resin Linked Derivatives of Serotonin and Affinity Chromatography of Proteins Isolated from Synaptic Membranes
Open Access
- 1 June 1974
- journal article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 29 (5-6) , 248-256
- https://doi.org/10.1515/znc-1974-5-612
Abstract
An affinity resin for the isolation of the serotonin receptor from nerve ending membranes was prepared by coupling tryptamine, 5-methoxy-tryptamine or serotonin to CNBr-activated Sepharose 4 B. The tryptamine derivatives, N-6-(aminohexyl)-tryptamines, were obtained by subsequent con densation of the corresponding indole derivatives with oxalyl chloride and t-aminocaproamide and reduction with LiAlH4. The 6-aminohexyl-group was used as spacer to link covalently the primary amino-group of tryptamine to agarose. - An extract of membrane proteins isolated from rat brain was resolved on this affinity resin into two fractions as compared with only one fraction using unsubstituted Sepharose 4 B.Keywords
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