Direct and sensitive detection of a human virus by rupture event scanning
- 1 September 2001
- journal article
- research article
- Published by Springer Nature in Nature Biotechnology
- Vol. 19 (9) , 833-837
- https://doi.org/10.1038/nbt0901-833
Abstract
We have developed a sensitive, economical method that directly detects viruses by making use of the interaction between type 1 herpes simplex virus (HSV1) and specific antibodies covalently attached to the oscillating surface of a quartz crystal microbalance (QCM). The virions were detached from the surface by monotonously increasing the amplitude of oscillation of the QCM, while using the QCM to sensitively detect the acoustic noise produced when the interactions were broken. We term this process rupture event scanning (REVS). The method is quantitative over at least six orders of magnitude, and its sensitivity approaches detection of a single virus particle.Keywords
This publication has 14 references indexed in Scilit:
- “Hearing” Bond Breakage. Measurement of Bond Rupture Forces Using a Quartz Crystal MicrobalanceLangmuir, 2000
- A silicon-based ultrasonic immunoassay for detection of breast cancer antigensSensors and Actuators B: Chemical, 1998
- Acoustic Wave Sensors and ResponsesPublished by Elsevier ,1997
- Experimental design for kinetic analysis of protein-protein interactions with surface plasmon resonance biosensorsJournal of Immunological Methods, 1997
- A novel immunosensor for herpes virusesAnalytical Chemistry, 1994
- In Situ Interfacial Mass Detection with Piezoelectric TransducersScience, 1990
- An Analysis of the Biological Properties of Monoclonal Antibodies against Glycoprotein D of Herpes Simplex Virus and Identification of Amino Acid Substitutions that Confer Resistance to NeutralizationJournal of General Virology, 1986
- A sensitive new method for the determination of adhesive bonding between a particle and a substrateJournal of Applied Physics, 1985
- The use of monoclonal antibodies to differentiate isolates of herpes simplex types 1 and 2 by neutralisation and reverse passive haemagglutination testsJournal of Medical Virology, 1984
- Electron microscopic particle counts on herpes virus using the phosphotungstate negative staining techniqueVirology, 1963