The Evaluation of Germicides by the Manometric Method

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Abstract
Manometric data on the effects of several germicides on the O2 consumption of Escherichia coli in a synthetic medium were compared with the lethal effects as shown by subsequent cultures. A complete inhibition of respiration was found necessary to render the organisms incapable of growth when subcultured. When germicides were added to suspensions of E. coli the percentage of inhibition of respiration increased to a maximum during each successive observation period. In no case did the effect recede from the maximum. The relation between the degree of inhibition of respiration and killing by sulfanilamide and by merthiolate differed in that the amt. of inhibition of respiration by sulfanilamide without a killing effect was greater than that for merthiolate. The presence of serum greatly inhibited the germicidal effect of merthiolate, tincture of iodine and sulphated castor oil containing sodium o-phenyl phenate, had little or no effect on sulfanilamide, and only a moderate effect on phenol and CH2O.

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