Measure of asymmetrical transcription of the yeast OMP decarboxylase gene expressed in yeast or in E. coli
- 1 October 1980
- journal article
- Published by Springer Nature in Current Genetics
- Vol. 2 (2) , 103-107
- https://doi.org/10.1007/bf00420621
Abstract
The yeast OMP decarboxylase gene has been cloned in the phage fd in both orientations to obtain pure DNA of each strand. With these probes the transcription of each strand of the yeast gene was measured in wild type yeast, either repressed or induced, in a yeast strain transformed by an hybrid plasmid containing the OMPdecase gene, and in E. coli strains bearing the yeast gene cloned in different plasmids. In yeast it appears that in all conditions tested transcription was asymmetrical, 95% being transcribed from one strand. In E. coli, transcription of the yeast gene occurred on both strands.Keywords
This publication has 12 references indexed in Scilit:
- Conversion of bacteriophage fd into an efficient single-stranded DNA vector systemMolecular Genetics and Genomics, 1980
- Interference of nonsense mutations with eukaryotic messenger RNA stability.Proceedings of the National Academy of Sciences, 1979
- Propagation of restriction fragments from the mitochondrial DNA of Saccharomyces cerevisiae in E coli by means of plasmid vectorsNucleic Acids Research, 1979
- Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.Proceedings of the National Academy of Sciences, 1977
- Transcription and translation of cloned Drosophila DNA fragments in Escherichia coliBiochemistry, 1977
- Studies of mouse mitochondrial DNA in escherichia coli: Structure and function of the eucaryotic-procaryotic chimeric plasmidsCell, 1975
- Mapping and characterization of promoters in bacteriophages fd, f1 and M13Journal of Molecular Biology, 1975
- Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purificationVirology, 1970
- Orotidine-5′-phosphate decarboxylase from higher plantsBiochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation, 1966
- A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membraneJournal of Molecular Biology, 1965