Microcalorimetric studies on metabolism of hepatic tissue II. Measurements of ischemic tissue

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Abstract
Summary Biopsies of ischemic livers demonstrated a significantly higher rate of heat production when incubated in Leibowitz L-15 medium compared to incubation in modified Eagle's solution (P<0.01). A maximal reduction of 25% in the rate of heat production was observed after 60 min of induced ischemia. Liver tissue undergoing reperfusion showed complete and partial recovery after 60 and 90 min, respectively. We conclude that the viability of liver tissue begins to detoriate after 1–1.5 h of ischemia. Using the level of adenosine triphosphate (ATP) as a parameter for viability, others had found this critical time period to be 1.5–2 h. Furthermore, we conclude that monitoring heat production of tissue provides a useful measure of viability for both clinical application and in connection with experimental liver surgery.