THE HETEROGENIZATION OF TUMOR-CELLS WITH TUBERCULIN .1. THE COUPLING OF TUBERCULIN TO CELL-SURFACES USING CON-A AS LIGAND

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  • 1 January 1981
    • journal article
    • research article
    • Vol. 42  (2) , 329-336
Abstract
The introduction of an antigenic determinant strongly recognized by T cells on to a cell enhances the immune response to weak cellular antigens. Tuberculin (PPD) is a particularly suitable antigenic determinant for this purpose since it behaves in many ways as a T-cell hapten. Direct chemical coupling of PPD to cell [mouse sarcoma B10] surfaces damages their antigenicity, but this can be circumvented by coupling PPD to the lectin and using this as the ligand for binding PPD to the cell. Techniques for preparing Con-A/PPD using little glutaraldehyde or N-succinyl 3-(2-pyridyldithio)propionate as cross-linking agents are described.