Changes in the ultrastructure of the parenchymal cell and in enzyme localization accompanying galactosamine-induced liver injury in rats.

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Abstract
Administration of galactosamine in large quantities to the rat induces liver injury which shows morphological and functional features similar to those of acute human viral hepatitis. Transient injury to the rat liver was induced by a single i.p. injection of galactosamine. After administration, most liver cells showed synchronous morphological alteration accompanied by changes in the histochemical localization of several enzymes in the liver cell. The histochemical reaction of sorbitol dehydrogenase at the light microscopic level was frequently restricted to 1 side of the cytoplasm and the peripheral cytoplasm near the cell border. At the EM level, the reaction deposits of sorbitol dehydrogenase were recognized in the hyaloplasm and its localization shifted with the movement of cell organelles.The localization of glucose-6-phosphatase changed with alteration of the shape of the endoplasmic reticulum and its distribution, while the localizations of 5''-nucleotidase and Mg2+-ATPase changed with the morphological alteration of the plasma membrane.